The fungal elicitor cryptogein is a sterol carrier protein

FEBS Letters

Blein, Jean-Pierre¹  Ponchet, Michel³  Ricci, Pierre³  Milat, Marie-Louise¹  Mikes, Vladimir² 

[1] Unité associée INRA/Université de Bourgogne 692, Laboratoire de Phytopharmacie et de Biochimie des Interactions Cellulaires, INRA, BV 1540, F-21034 Dijon Cedex, France;Department of Biochemistry, Faculty of Science, Masaryk University, Kotlarska 2, 61137 Brno, Czech Republic;INRA Station de Botanique et Pathologie Végétale, BP2078, F-06606 Antibes Cedex, France

关键词: Dehydroergosterol;    Elicitin;    Fluorescence;    Phytophthora;    DHE;    dehydroergosterol;    SUV;    small unilamellar vesicles;    PC;    phosphatidylcholine;    PS;    phosphatidylserine;    BSA;    bovine serum albumin;    SCP;    sterol carrier protein;   

DOI  :  10.1016/S0014-5793(97)01193-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Cryptogein is a protein secreted by the phytopathogenic pseudo-fungus, Phytophthora cryptogea. It is a basic 10 kDa hydrophilic protein having a hydrophobic pocket and three disulfide bridges. These common features with sterol carrier proteins led us to investigate its possible sterol transfer activity using the fluorescent sterol, dehydroergosterol. The results show that cryptogein has one binding site with strong affinity for dehydroergosterol. Moreover, this protein catalyzes the transfer of sterols between phospholipidic artificial membranes. This is the first evidence for the existence of an extracellular sterol carrier protein and for a molecular activity of cryptogein. This property should contribute to an understanding of the role of cryptogein in plant-microorganism interactions.

【 授权许可】

Unknown