Class 2 aldehyde dehydrogenase. Characterization of the hamster enzyme, sensitive to daidzin and conserved within the family of multiple forms

FEBS Letters

Keung, Wing Ming¹  Norin, Annika²  Klyosov, Anatole¹  Hjelmqvist, Lars²  Vallee, Bert¹  Lundgren, Robert²  Jörnvall, Hans² 

[1] Center for Biochemical and Biophysical Sciences and Medicine, Harvard Medical School, 250 Longwood Ave, Boston, MA 02115, USA;Department of Medical Biochemistry and Biophysics, Karolinska Institutet, S-171 77 Stockholm, Sweden

关键词: Aldehyde dehydrogenase;    Variability pattern;    Evolutionary change;    Residue difference;    Conserved enzyme;   

DOI  :  10.1016/S0014-5793(97)01176-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Mitochondrial (class 2) hamster aldehyde dehydrogenase has been purified and characterized. Its primary structure has been determined and correlated with the tertiary structure recently established for this class from another species. The protein is found to represent a constant class within a complex family of multiple forms. Variable segments that occur in different species correlate with non-functional segments, in the same manner as in the case of the constant class of alcohol dehydrogenases (class III type) of another protein family, but distinct from the pattern of the corresponding variable enzymes. Hence, in both these protein families, overall variability and segment architectures behave similarly, with at least one `constant' form in each case, class III in the case of alcohol dehydrogenases, and at least class 2 in the case of aldehyde dehydrogenases.

【 授权许可】

Unknown