Trifluoroethanol‐induced conformational transition of hen egg‐white lysozyme studied by small‐angle X‐ray scattering

FEBS Letters

Hamada, Daizo²  Kataoka, Mikio¹  Goto, Yuji²  Hoshino, Masaru²  Hagihara, Yoshihisa² 

[1] Department of Earth and Space Science, Graduate School of Science, Osaka University, Toyonaka, Osaka 560, Japan;Department of Biology, Graduate School of Science, Osaka University, Toyonaka, Osaka 560, Japan

关键词: α-Helix;    Circular dichroism;    Lysozyme;    Protein folding;    Solution X-ray scattering;    Trifluoroethanol;    CD;    circular dichroism;    Rcm;    disulfide bond-reduced and carboxymethylated;    SAXS;    small-angle X-ray scattering;    SVD;    singular value decomposition;    TFE;    2;    2;    2-trifluoroethanol;   

DOI  :  10.1016/S0014-5793(97)01172-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The trifluoroethanol (TFE)-induced conformational transition of hen lysozyme was studied with the combined use of far-UV circular dichroism (CD) and small-angle X-ray scattering. At pH 2.0 and 20°C, the addition of TFE to the native lysozyme induced a cooperative transition to an intermediate state with an increased helical content (TFE state). Small-angle X-ray scattering measurements indicated that the TFE state has a radius of gyration which is 20% larger than that of the native state and assumes a chain-like conformation with some remaining globularity. The TFE-induced transition curves obtained by CD and the small-angle X-ray scattering measurements agreed well, consistent with a two-state transition mechanism. A singular value decomposition analysis of Kratky plots of the small-angle X-ray scattering profiles indicated that two basic scattering functions reproduce the observed spectra, further confirming the validity of a two-state approximation.

【 授权许可】

Unknown