The tomato RBCS3A promoter requires integration into the chromatin for correct organ‐specific regulation

FEBS Letters

Michalowski, Susan¹  Gröning, Birge²  Meier, Iris²  Spiker, Steven¹ 

[1] Department of Genetics, North Carolina State University, Raleigh, NC 27695, USA;Institute for General Botany, AMP I, University of Hamburg, Ohnhorststr. 18, D-22609 Hamburg, Germany

关键词: Chromatin;    Matrix attachment region;    Organ-specific;    RBCS promoter;    Tomato;   

DOI  :  10.1016/S0014-5793(97)01102-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

In tomato, the RBCS1, RBCS2 and RBCS3A genes, encoding the small subunit of ribulose-1,5-bisphosphate carboxylase/oxygenase, are expressed in leaves and light-grown seedlings, but only RBCS1 and RBCS2 are expressed in developing tomato fruits. The activities of the three promoters have been compared in transgenic plants and after transient transformation. Fruit-specific repression of the RBCS3A promoter was observed in transgenic plants, but not after ballistic transient transformation, indicating that chromatin integration is necessary for its correct organ-specific regulation. In addition, matrix attachment regions have been identified in the RBCS1, RBCS2 and RBCS3A promoters. This is the second case in plants of absence of correct regulation of a plasmid-borne plant promoter and correlating potential nuclear matrix attachment of the gene.

【 授权许可】

Unknown