期刊论文详细信息
FEBS Letters
Cytochrome b 6/f complex from the cyanobacterium Synechocystis 6803: evidence of dimeric organization and identification of chlorophyll‐binding subunit
Barbato, Roberto1  Poggese, Chiara1  Giacometti, Giorgio M1  Polverino de Laureto, Patrizia2  Rigoni, Fernanda1 
[1] Dipartimento di Biologia, Università di Padova, Via Trieste 75, 35121 Padua, Italy;CRIBI, Università di Padova, Via Trieste 75, 35121 Padua, Italy
关键词: Cytochrome b 6/f;    Pigment-binding protein;    Dimeric/monomeric organization;    Synechocystis 6803;    Deriphat;    N-lauryl iminodipropionate;    disodium salt;    FeS protein;    iron-sulfur Rieske protein;    TMBZ;    N;    N;    N′;    N′-tetramethylbenzidine;   
DOI  :  10.1016/S0014-5793(97)01078-8
学科分类:生物化学/生物物理
来源: John Wiley & Sons Ltd.
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【 摘 要 】

Fractionation of photosynthetic membranes from the cyanobacterium Synechocystis 6803 by polyacrylamide gel electrophoresis in the presence of Deriphat-160 allowed the isolation of a number of pigmented bands. Two of them, with molecular masses of 240±20 and 110±15 kDa respectively, showed peroxidase activity and, by means of polypeptide composition, immunoblotting and N-terminal sequencing, were identified as dimeric and monomeric cytochrome b 6/f complexes, containing 1.3±0.35 chlorophyll molecules per cytochrome f. Further fractionation of monomeric complexes by mild gel electrophoresis in the presence of sodium dodecyl sulfate indicated that it is the cytochrome b 6 polypeptide which provides the actual binding site for the chlorophyll molecule observed in the complex.

【 授权许可】

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