Properties of the extracellular calcium binding module of the proteoglycan testican

FEBS Letters

Maurer, Patrik¹  Vannahme, Christian¹  Kohfeldt, Eddie²  Timpl, Rupert² 

[1] Institut für Biochemie II der Medizinischen Fakultät, Universität Köln, D-60924 Cologne, Germany;Max-Planck-Institut für Biochemie, D-82152 Martinsried, Germany

关键词: Calcium binding;    EF hand;    Extracellular matrix;    Recombinant protein;   

DOI  :  10.1016/S0014-5793(97)01070-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The extracellular calcium-binding (EC) module of human testican (115 residues) was obtained in native form by recombinant production in mammalian cell culture and thus shown to represent an independently folding domain. This module showed a large loss in α-helix upon calcium depletion. Apparently only one of the two EF hands binds calcium, with a moderate affinity (K _d=68 μM) about 100-fold lower than in the homologous BM-40 protein. No clear evidence was obtained for collagen binding, indicating that EC modules found in different proteins may not share similar functions.

【 授权许可】

Unknown