Prediction of substrate‐specific pockets in cyclosporin synthetase

FEBS Letters

Schörgendorfer, Kurt²  Taylor, Paul¹  Husi, Holger¹  Walkinshaw, Malcolm D¹  Stempfer, Günter² 

[1] Structural Biochemistry Group, Department of Biochemistry, The University of Edinburgh, Michael Swann Building, King's Buildings, Edinburgh EH9 3JR, UK;Biochemie GmbH, 6330 Kufstein-Schaftenau, Austria

关键词: Cyclosporin A synthetase;    Comparative sequence homology alignment;    Active site prediction;   

DOI  :  10.1016/S0014-5793(97)01064-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Amino acid sequence comparisons between domains of cyclosporin synthetase have been used to identify regions of the sequence which are responsible for the recognition and binding of the individual amino acids. Using a limited set of selection rules it was possible to identify three amino acid positions in the subdomain sequences which are responsible for amino acid specificity. Homology with the firefly luciferase protein shows that these three key residues are close to each other and line the surface of a putative specific substrate binding pocket located on the amino acyl-adenylation subdomain. These results allow us to predict a large number of cyclosporin synthetase mutants which could be used to synthesise alternative cyclosporin-like peptides.

【 授权许可】

Unknown