Growth stage dependent effects of electromagnetic fields on DNA synthesis of Jurkat cells

FEBS Letters

Balcavage, W.X²  Nindl, G²  Miller, J.M²  Swez, J.A¹ 

[1] Department of Physics, Indiana State University, Terre Haute, IN 47809, USA;Indiana University School of Medicine at Indiana State University, Terre Haute Center For Medical Education, Holmstedt Hall 135, Terre Haute, IN 47809, USA

关键词: Electromagnetic field;    DNA synthesis;    Growth;    T-lymphocyte;    Jurkat T-cell line;    Ca2+;    calcium;    EMF;    electromagnetic field;    PBL;    peripheral blood lymphocyte;    T;    Tesla;   

DOI  :  10.1016/S0014-5793(97)01060-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A 1.8 mT, bone healing, electromagnetic field (EMF) and power frequency EMFs of 0.1 and 0.4 mT significantly inhibit DNA synthesis in otherwise unstimulated Jurkat (E 6.1) cells. Inhibition is generally most prominent in cells from mid log phase growth. In complete medium the bone healing EMF inhibits [³H] thymidine uptake of the latter cells by almost 50% vs. 20–25% inhibition by 60 Hz fields. Cells in conditioned medium are even more sensitive to EMFs with inhibition up to ca. 60%. The effects of the 0.1 and 0.4 mT power frequency EMFs were very similar suggesting saturation at 0.1 mT or lower.

【 授权许可】

Unknown