Expression of arachidonate platelet‐type 12‐lipoxygenase in human rheumatoid arthritis type B synoviocytes

FEBS Letters

Liagre, Bertrand¹  Rigaud, Michel¹  Vergne, Pascale²  Beneytout, Jean L¹ 

[1] Facultés de Médecine et de Pharmacie, ERS CNRS 6101, 2 Rue du Docteur Marcland, 87025 Limoges Cedex, France;Service de Rhumatologie, CHRU de Limoges, Hôpital Dupuytren, 2 Avenue Martin Luther King, 87042 Limoges Cedex, France

关键词: Rheumatoid arthritis;    Human synoviocyte;    12-Lipoxygenase;    Arachidonic acid;    Cytokine;   

DOI  :  10.1016/S0014-5793(97)00904-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

In the present study, we have demonstrated platelet-type 12-lipoxygenase (12-LOX) expression in human rheumatoid arthritis (RA) type B synoviocytes by reverse-transcription polymerase chain reaction (RT-PCR). The presence of 12-LOX mRNA in these cells was revealed by classical RT-PCR analysis using platelet-type 12-LOX cDNA primers and the PCR fragment (246 bp) was purified, amplified and sequenced. By sequence analysis, this fragment was determined to be 100% identical to that from platelet-type 12-LOX cDNA. Immunofluorescence data demonstrate that interleukin-1β (IL-1β) increases cellular 12-LOX protein. Other results associate specific inflammatory cytokines with the activity of 12-LOX in human RA type B synoviocytes. IL-1β increased 12S-hydroxy-5,8,10,14-eicosatetraenoic acid (12-HETE) production (4-fold) and we also observed an increase in 12-HETE production (2.5-fold) after incubation of human RA type B synoviocytes with TNFα. In contrast to the action of IL-1β on 12-HETE synthesis, IL-4 and IL-6 did not enhance 12-HETE production. This is the first demonstration of platelet-type 12-LOX cDNA derived from the mRNA of cultured human RA type B synoviocytes.

【 授权许可】

Unknown