The assimilatory nitrate reductase from the phototrophic bacterium, Rhodobacter capsulatus E1F1, is a flavoprotein

FEBS Letters

Blasco, R¹  Martı́nez-Luque, M¹  Castillo, F¹ 

[1] Departamento de Bioquı́mica y Biologı́a Molecular, Facultad de Ciencias, Universidad de Córdoba, 14071 Córdoba, Spain

关键词: Nitrate reductase;    Flavoprotein;    Phototrophic bacterium;    p-HMB;    p-hydroxymercuribenzoate;    BPB(H);    (reduced) bromophenol blue;    DCPIP;    dichlorophenol indophenol;    MV;    methyl viologen;    MTA;    mixed alkyl trimethylammonium bromide;   

DOI  :  10.1016/S0014-5793(97)00968-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The assimilatory nitrate reductase from the phototrophic bacterium Rhodobacter capsulatus has been purified to electrophoretic homogeneity and its molecular and kinetic parameters determined. The native nitrate reductase is a dimer of 144 kDa composed of two subunits of 46 and 95 kDa. The purified enzyme catalyzes the electron transfer from NADH, reduced bromophenol blue or reduced viologens to nitrate. The nitrate reductase contains 1 mol FAD per mole of enzyme and also reduces cytochrome c or dichlorophenol indophenol with NADH as the electron donor. The diaphorase activity is located in the small subunit.

【 授权许可】

Unknown