Antibodies to steroids from a small human naive IgM library

FEBS Letters

Kürschner, Timo²  Rohrbach, Petra²  Little, Melvyn²  Braunagel, Michael²  Welschof, Martin¹  Dörsam, Heinz²  Renner, Stefanie²  Kipriyanov, Sergey² 

[1] Inst. Immunology, Dept. Transplantation Immunology, University of Heidelberg, INF305, 69120 Heidelberg, Germany;German Cancer Research Center, Recombinant Antibodies (0445), Diagnostics and Experimental Therapy Programme (4), INF 280, 69120 Heidelberg, Germany

关键词: Antibody library;    Human IgM repertoire;    Single chain antibody;    Anti-steroid antibody;    Phage display;   

DOI  :  10.1016/S0014-5793(97)00966-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Human antibodies specific for digoxigenin, estradiol, testosterone and progesterone have been isolated from a small combinatorial IgM repertoire (4×10⁷) of single chain antibodies (scFv). The affinities of both the anti-estradiol and anti-progesterone scFv were approximately 10⁸ M⁻¹. Naive IgM genes appeared to be highly represented, since only the heavy chain variable domain of the anti estradiol antibody contained differences to corresponding germline sequences. The light chain variable domain of the progesterone receptor was also identical to a germline sequence, showing that it is possible for completely naive antibodies to bind steroids with affinities comparable to those obtained after a secondary immune response.

【 授权许可】

Unknown