Human serotonin 5‐HT7 receptor: cloning and pharmacological characterisation of two receptor variants

FEBS Letters

Dijcks, Fred¹  Olijve, Wiebe²  Roesink, Carolien²  van Herpen, Anne²  Garritsen, Anja¹  Stam, Nico J² 

[1] Organon N.V., Department of Neuropharmacology, P.O. Box 20, 5340 BH Oss, The Netherlands;Organon N.V., Department of Biotechnology and Biochemistry, P.O. Box 20, 5340 BH Oss, The Netherlands

关键词: G-protein-coupled receptor;    Receptor variant;    5-HT;    Alternative splicing;   

DOI  :  10.1016/S0014-5793(97)00964-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Two splice variants of the 5-HT₇ receptor were identified in human brain that differ in the lengths of their intracellular carboxy terminal tail. Identification of the variants of this receptor is of particular interest since the 5-HT₇ receptor is known to have a high affinity for a number of antidepressants and is localized in brain regions thought to be implicated in depression. The two isoforms are expressed in roughly equal amounts in various regions of the human brain. When expressed in NIH-3T3 cells, both variants encode functional 5-HT₇ receptors, positively coupled to adenylyl cyclase. We suggest that both variants are derived from a single gene by alternative mRNA splicing. Furthermore, our results from Southern blot analysis studies suggest that additional 5-HT₇ receptor genes may exist in human.

【 授权许可】

Unknown