Proadrenomedullin N‐terminal 20 peptide (PAMP) inhibits proliferation of human neuroblastoma TGW cells

FEBS Letters

Shimosawa, Tatsuo¹  Ando, Katsuyuki¹  Fujita, Toshiro¹  Omi, Naomi¹ 

[1]Fourth Department of Internal Medicine, University of Tokyo School of Medicine, 3-28-6 Mejirodai, Bunkyo-ku, Tokyo 112, Japan

关键词: Adrenomedullin;    Pertussis toxin;    Calcitonin gene-related peptide;    Cell growth;    ω-Conotoxin GIVA;    AM;    adrenomedullin;    ANOVA;    analysis of variance;    BrdU;    5-bromo-2′-deoxyuridine;    CGRP;    calcitonin gene-related peptide;    FBS;    fetal bovine serum;    MoAb-G6;    polyclonal antibody against P072;    a fragment of AM;    MTT;    3-[4;    5-dimethylthiazol-2-yl]-2;    5-diphenyltetrazolium bromide;    PAMP;    proadrenomedullin N-terminal 20 peptide;    PTX;    pertussis toxin;    TMB-8;    8-[N;    N-dimethlyamino]octyl-3;    4;    5-trimethoxybenzoate hydrochloride;    VSMC;    vascular smooth muscle cells;    ω-CTX;    ω-conotoxin GIVA;   

DOI  :  10.1016/S0014-5793(97)00958-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We investigated the effects of proadrenomedullin N-terminal 20 peptide (PAMP) and adrenomedullin (AM) on the growth of human neuroblastoma TGW cells. Both PAMP and AM inhibited growth and DNA synthesis in neuroblastoma cells. Calcitonin gene-related peptide (CGRP)_8–37, an antagonist to CGRP, abolished the inhibitory effect of AM on growth and DNA synthesis of neuroblastoma cells but did not affect that of PAMP. AM_22–52, an antagonist to AM, also reversed the effect of AM. On the other hand, pertussis toxin (PTX) and ω-conotoxin GIVA blocked the effect of PAMP alone. Thus, PAMP inhibits the growth of neuroblastoma cells by inhibiting N-type Ca²⁺ channels through PTX-sensitive G protein-coupled receptors, which is different mechanism of AM-induced inhibition of the cell growth.

【 授权许可】

Unknown