The cytochrome oxidase subunit 1 gene (cox1) from the dinoflagellate, Crypthecodinium cohnii

FEBS Letters

Norman, John E¹  Gray, Michael W¹ 

[1] Program in Evolutionary Biology, Canadian Institute for Advanced Research, Department of Biochemistry, Dalhousie University, Halifax, Nova Scotia B3H 4H7, Canada

关键词: Cytochrome oxidase;    Alveolate;    Codon usage;    Molecular phylogeny;    Evolution;    (Crypthecodinium cohnii);    kbp;    103 base pairs;    nt;    nucleotide;    bp;    base pair;    PCR;    polymerase chain reaction;    rRNA;    ribosomal RNA;    LSU;    large subunit;    SSU;    small subunit;    SDS;    sodium dodecyl sulphate;    EDTA;    ethylenediaminetetraacetate;    DTT;    dithiothreitol;    dNTP;    deoxynucleoside triphosphate;   

DOI  :  10.1016/S0014-5793(97)00938-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

To date, no genes have been characterized from dinoflagellate mitochondrial DNA. Here we present the complete sequence of the gene (cox1) encoding subunit 1 of cytochrome c oxidase in the dinoflagellate, Crypthecodinium cohnii. Analysis of nucleotide and deduced amino acid sequences predicts a protein of 523 amino acids that is translated using universal initiation, stop and tryptophan codons. COX1 amino acid identity and phylogenetic tree analyses strongly support a close evolutionary relationship between dinoflagellates and apicomplexans; however, inclusion of the ciliates in this clade is less well supported, a result likely due to the highly derived nature of ciliate COX1 sequences.

【 授权许可】

Unknown