Isolation and characterization of mutations affecting expression of the Δ9‐ fatty acid desaturase gene, OLE1, in Saccharomyces cerevisiae

FEBS Letters

Sugioka, Shigemi²  Anamnart, Sarintip²  Harashima, Satoshi²  Fujimori, Ko³  Nakagawa, Youji²  Oshima, Yasuji¹  Ohta, Daisaku³  Yamada, Yasuhiro² 

[1] Department of Biotechnology, Faculty of Engineering, Kansai University, 3-3-35 Yamatecho, Suita-shi, Osaka 564, Japan;Department of Biotechnology, Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita-shi, Osaka 565, Japan;Takarazuka Research Institute, Novartis Pharma K.K., 10-66, Miyuki-cho, Takarazuka 665, Japan

关键词: Fatty acid desaturase;    Mutation;    OLE1;    Regulation;    (Saccharomyces cerevisiae);   

DOI  :  10.1016/S0014-5793(97)00846-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Expression of the Δ9- fatty acid desaturase gene, OLE1, of Saccharomyces cerevisiae is negatively regulated transcriptionally and post-transcriptionally by unsaturated fatty acids. In order to isolate mutants exhibiting irregulation of OLE1 expression, we constructed an OLE1p–PHO5 fusion gene as a reporter consisting of the PHO5 gene encoding repressible acid phosphatase (rAPase) under the control of the OLE1 promoter (OLE1p). By EMS mutagenesis, we isolated three classes of mutants, pfo1, pfo2 and pfo3 (

【 授权许可】

Unknown