Vinculin gene is non‐essential in Drosophila melanogaster

FEBS Letters

Alatortsev, Vladimir E¹  Kramerova, Irina A¹  Westphal, Elena D¹  Lavrov, Sergey A¹  Frolov, Maxim V¹ 

[1] Institute of Molecular Genetics, Russian Academy of Sciences, Kurchatov Sq. 46, Moscow 123182, Russia

关键词: Protein homology;    Vinculin function;    Chromosome rearrangement;   

DOI  :  10.1016/S0014-5793(97)00901-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Vinculin is thought to be an important cytoskeletal protein in the linkage between actin cytoskeleton and integrin transmembrane receptors. We identified Vinculin (Vinc) gene in the X chromosome of D. melanogaster. Drosophila vinculin is highly homologous in its N- and C-terminal domains both to mammalian and nematode vinculins, and contains internal repeats and proline-rich region typical for vinculins. The X chromosome rearrangement In(1LR)pn2a was found to disrupt Vinc so that the coding sequence is interrupted by the (AAGAG) _n satellite DNA. Northern analysis revealed that the Vinc transcript is completely absent in the In(1LR)pn2a homozygous flies. Surprisingly, these Vinc flies are viable and fertile. This finding highlights plasticity and adaptive capacity of cellular cytoskeletal and anchorage system.

【 授权许可】

Unknown