Essential role of the first intron in the transcription of hsp90β gene

FEBS Letters

Cheng, Xiao-kuan¹  Liu, Ju-hong²  Wang, Yan-lin²  Shen, Yu-fei¹  Wang, Xiao-zhe²  Wu, Ning-hua¹ 

[1] National Laboratory of Medical Molecular Biology, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing, China;Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing, China

关键词: hsp90β gene;    Heat shock element (HSE);    The first intron;    Transcription initiation site;    Chloramphenicol acetyl transferase (CAT);   

DOI  :  10.1016/S0014-5793(97)00883-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The human HSP90 gene family contains introns. There are two typical heat shock elements (HSE) in the first intron of human hsp90β gene. As detected by chloramphenicol acetyl transferase (CAT) reporter activity assays, the HSE-containing intron is essential in maintaining high constitutive expression and is critical for heat shock inducibility of the human hsp90β gene. Cellular heat shock factor 1 (HSF 1) shows much higher binding affinity toward the intronic HSEs in comparison to an atypical HSE in the 5′ flanking sequence. Novel initiation sites found in the first intron probably also contribute to constitutive and heat-inducible expression of the hsp90β gene in Jurkat cells.

【 授权许可】

Unknown