Burdock, a novel retrotransposon in Drosophila melanogaster, integrates into the coding region of the cut locus

FEBS Letters

Anashchenko, V.A.¹  Ponomarenko, N.A.¹  Bannikov, V.M.¹  Tchurikov, N.A.¹ 

[1] Engelhardt Institute of Molecular Biology, Russian Academy of Science, Vavilov str. 32, Moscow 117334, Russia

关键词: Mobile element;    burdock;    cut locus;    Topoisomerase II;    Drosophila melanogaster;   

DOI  :  10.1016/S0014-5793(97)00844-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The burdock element is known to be the 2.6-kb insertion into the same region of the cut locus in 12 independently obtained ct-lethal mutants. Here we have determined the complete sequences of this insertion and of the hot spot region. It was found that the burdock is a short retrotransposon with long terminal repeats and a single open reading frame (ORF). The polypeptide encoded by the burdock ORF contains two adjacent regions homologous to the gag and pol polyproteins of the gypsy mobile element. The burdock insertion interrupts the short ORF of the cut locus. The target site sequence of the burdock insertions is similar to the Drosophila topoisomerase II cleavage site.

【 授权许可】

Unknown