期刊论文详细信息
FEBS Letters | |
K+ is an indispensable cofactor for GrpE stimulation of ATPase activity of DnaK·DnaJ complex from Thermus thermophilus | |
Yohda, Masafumi1  Motohashi, Ken2  Odaka, Masafumi1  Yoshida, Masasuke2  | |
[1] The Institute of Physical and Chemical Research (RIKEN), Hirosawa 2-1, Wako-shi, Saitama 351-01, Japan;Research Laboratory of Resources Utilization, R-1, Tokyo Institute of Technology, Nagatsuta 4259, Yokohama 226, Japan | |
关键词: DnaK; DnaJ; GrpE; Heat-shock protein; Hsp70; Molecular chaperone; Hsp70; heat-shock protein of 70 kDa; T.DnaK·DnaJ complex; DnaK·DnaJ complex of T. thermophilus; T.GrpE; GrpE of T. thermophilus; | |
DOI : 10.1016/S0014-5793(97)00847-8 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
K+ is an indispensable cofactor for ATPase activity of eukaryotic cytosolic Hsp70 chaperone systems which lack a GrpE homolog. In the case of the bacterial Hsp70 (DnaK) system, GrpE, a nucleotide exchange factor, stimulates ATPase activity but little is known about the effect of K+. Here, we have cloned a grpE gene from a thermophile, Thermus thermophilus, and purified a homodimeric GrpE protein. Using proteins of this bacterium, we found that the GrpE stimulation of ATPase activity of DnaK·DnaJ complex was absolutely dependent on the presence of K+.
【 授权许可】
Unknown
【 预 览 】
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