Involvement of HNF‐1 in the regulation of phosphoenolpyruvate carboxykinase gene expression in the kidney

FEBS Letters

Nechushtan, Hovav¹  Hanson, Richard W²  Reshef, Lea¹  Livoff, Alejandro F¹  Cassuto, Hanoch¹  Olswang, Yael¹ 

[1] Department of Developmental Biochemistry, Hebrew University-Hadassah Medical School, P.O. Box 12272, Jerusalem 91120, Israel;Department of Biochemistry, School of Medicine, Case Western Reserve University, Cleveland, Ohio, USA

关键词: PEPCK;    Kidney;    Transcription;    Basal;    Modulation;    HNF-1;    PEPCK;    phosphoenolpyruvate carboxykinase (GTP) (EC 4.1.1.32);    CRE;    cAMP response element;    HNF-1;    hepatic nuclear factor-1;    C/EBP;    CCAAT/enhancer binding protein;    kbp;    kilobase pair(s);    bp;    base pair(s);    rp;    ribosomal proteins;    hGH;    human growth hormone;    bGH;    bovine growth hormone;    SV40;    simian virus 40;    RSV;    Rous Sarcoma virus;    DMEM;    Dulbecco Modified Eagle's medium;    CAT;    chloramphenicol acetyltransferase;    PPAR;    peroxisome proliferator-activated receptor;   

DOI  :  10.1016/S0014-5793(97)00867-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The cytosolic form of phosphoenolpyruvate carboxykinase (GTP) (PEPCK) gene is differentially expressed in several tissues. A specific set of regulatory elements in the promoter are responsible for the control of PEPCK gene transcription and, in turn, determine its distinct metabolic role in each tissue. DNase I footprinting analysis of the PEPCK promoter, using nuclear proteins from tissues which express the gene for PEPCK, and transient expression assays in renal cell lines have demonstrated that the HNF-1 recognition motif (P2) in the PEPCK promoter characterizes kidney-specific expression. This site is required also for the response to acidosis. Since the P2 site is not involved in the expression of the PEPCK gene in the liver, we propose that its critical role in the kidney stems from a combination of abundance of HNF-1 together with low concentrations of members of the C/EBP family in this tissue.

【 授权许可】

Unknown