9‐cis‐Retinoic acid enhances fatty acid‐induced expression of the liver fatty acid‐binding protein gene

FEBS Letters

Niot, I¹  Wahli, W²  Poirier, H¹  Besnard, P¹  Braissant, O² 

[1] Laboratoire de Physiologie de la Nutrition, E.A. DRED 580, Ecole Nationale Supérieure de Biologie Appliquée à la Nutrition et à l'Alimentation (ENSBANA), Université de Bourgogne, I, Esplanade Erasme, 21000 Dijon, France;Institut de Biologie Animale, Université de Lausanne, Bâtiment de Biologie, CH-1015 Lausanne, Switzerland

关键词: Liver fatty acid-binding protein;    9-cis-Retinoic acid;    Fatty acid;    Nuclear receptor;    Gene expression;    FAO hepatoma cell;    L-FABP;    liver fatty acid-binding protein;    ACO;    acyl-CoA oxidase;    BFE;    bifunctional enzyme;    RA;    retinoic acids;    9-cis-RA;    9-cis-retinoic acid;    FA;    fatty acid;    LCFA;    long chain fatty acid;    PPAR;    peroxisome proliferator-activated receptor;    FAAR;    fatty acid-activated receptor;    RXR;    retinoid X receptor;    PPRE;    peroxisome proliferator-responsive element;   

DOI  :  10.1016/S0014-5793(97)00830-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The role of retinoic acids (RA) on liver fatty acid-binding protein (L-FABP) expression was investigated in the well differentiated FAO rat hepatoma cell line. 9-cis-Retinoic acid (9-cis-RA) specifically enhanced L-FABP mRNA levels in a time- and dose-dependent manner. The higher induction was found 6 h after addition of 10⁻⁶ M 9-cis-RA in the medium. RA also enhanced further both L-FABP mRNA levels and cytosolic L-FABP protein content induced by oleic acid. The retinoid X receptor (RXR) and the peroxisome proliferator-activated receptor (PPAR), which are known to be activated, respectively, by 9-cis-RA and long chain fatty acid (LCFA), co-operated to bind specifically the peroxisome proliferator-responsive element (PPRE) found upstream of the L-FABP gene. Our result suggest that the PPAR-RXR complex is the molecular target by which 9-cis-RA and LCFA regulate the L-FABP gene.

【 授权许可】

Unknown