Differential expression of mRNAs for endopeptidases in phenotypically modulated (`dedifferentiated') human articular chondrocytes

FEBS Letters

Baici, Antonio²  Kostoulas, Georgios²  Lang, Angela²  Trueb, Beat¹ 

[1] M.E. Müller-Institute of Biomechanics, University of Bern, P.O. Box 30, CH-3010 Bern, Switzerland;University Hospital, Department of Rheumatology, CH-8091 Zurich, Switzerland

关键词: Cathepsin B;    Cathepsin L;    Collagenase-1;    Stromelysin-1;    TIMP-2;    Osteoarthritis;    GAPDH;    glyceraldehyde 3-phosphate dehydrogenase;   

DOI  :  10.1016/S0014-5793(97)00825-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Human articular chondrocytes modulated away from their original phenotype by serial subcultures in monolayer differentially express mRNAs for endopeptidases. The mRNAs for the cathepsins B and L are extremely low in differentiated cells, but are soon expressed in parallel with the loss of the differentiated state. In contrast, the mRNA for collagenase-1 is strongly expressed by differentiated chondrocytes and declines rapidly following phenotypic modulation. The mRNA for stromelysin-1 and the tissue inhibitor of metalloproteinases-2 is high and does not appreciably change after modulation. Chondrocyte activation induced by alteration of its original phenotype leads to the expression of endopeptidases in a way that markedly differs from that induced by cytokines. The results are relevant to cartilage catabolism in osteoarthritis and suggest a prominent role of fibroblastic metaplasia on the part of the chondrocytes as a mechanism of expressing catabolic endopeptidases.

【 授权许可】

Unknown