A simple method for determining kinetic constants of complexing inactivation at identical enzyme and inhibitor concentrations

FEBS Letters

Wang, Ming-Hua¹  Zhao, Kang-Yuan¹ 

[1] National Laboratory of Biomacromolecules, Institute of Biophysics, Academia Sinica, Beijing 100101, People's Republic of China

关键词: Tight-binding;    complexing inactivation;    Kinetic constant;    Progress curve;   

DOI  :  10.1016/S0014-5793(97)00771-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The kinetics of complexing inactivation at identical enzyme and inhibitor concentrations were analyzed and the equations of product generation were derived when the free enzyme concentration is great, larger or smaller than the dissociation constant of inhibitor, K _I. The kinetic constants can be obtained by fitting the derived equations to the progress curve. Numerical examples show that this method is valid and gives satisfactory results.

【 授权许可】

Unknown