The presence of a transcription activation function in the hormone‐binding domain of androgen receptor is revealed by studies in yeast cells

FEBS Letters

Jänne, O.A¹  Rouleau, N¹  Moilanen, A¹  Palvimo, J.J¹  Ikonen, T¹ 

[1] Department of Physiology, Institute of Biomedicine, University of Helsinki, FIN-00014 Helsinki, Finland

关键词: Androgen receptor;    Transcription;    Ligand-binding domain;    Yeast;   

DOI  :  10.1016/S0014-5793(97)00791-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

To assess the importance of various regions of the androgen receptor (AR) in transcriptional regulation, we have compared its activation functions (AFs) in yeast and mammalian cells. The receptor's amino-terminal region contains a major transcriptional activator (AF-1) in both cell types, whereas AF-2 in the ligand-binding domain (LBD) is very weak in mammalian cells but clearly functional in the yeast. Hormone-binding ability of LBD is mandatory for AF-2 to operate, as illustrated by mutated LBD constructs. The activity of AF-2 in yeast is severely attenuated when the hinge region is attached to LBD, suggesting that the former region modulates AF-2 in vivo, probably by presenting an interface for interacting proteins.

【 授权许可】

Unknown