Unique protein kinase C profile in mouse oocytes: lack of calcium‐dependent conventional isoforms suggested by rtPCR and Western blotting

FEBS Letters

Jones, Keith T¹  Gangeswaran, Rathi¹ 

[1] Medical Research Council Experimental Embryology and Teratology Unit, St. George's Hospital Medical School, London, SW17 0RE, UK

关键词: Protein kinase C;    Oocyte;    Calcium;    Fertilization;    Mouse;    PCR;    DAG;    diacylglycerol;    GV;    germinal vesicle;    hCG;    human chorionic gonadotrophin;    IP3;    inositol trisphosphate;    M2;    metaphase II;    PBS;    phosphate-buffered saline;    PKC;    protein kinase C;    PVA;    polyvinyl alcohol;   

DOI  :  10.1016/S0014-5793(97)00782-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

rtPCR and Western blotting were used to determine which members of the PKC family are present in both immature and mature mouse oocytes. Using isoform-specific PCR primers and antibodies PKC-δ and -λ were detected while such techniques failed to observe the conventional isoforms of PKC-α, -β, -γ. This isoform profile was confirmed using an alternative PCR strategy, which allowed discrimination of PCR products derived from conventional and novel PKC isoforms. In addition PKC-ϵ, -η, -θ and -ζ were not detected by rtPCR. These results suggest that the predominant isoforms in oocytes are PKC-δ and -λ.

【 授权许可】

Unknown