Measurement of gluconeogenesis and pyruvate recycling in the rat liver: a simple analysis of glucose and glutamate isotopomers during metabolism of [1,2,3‐13C3]propionate

FEBS Letters

Cottam, G.Larry²  Jeffrey, F.M.H.¹  Jones, John G.¹  Naidoo, Randy¹  Sherry, A.Dean¹  Malloy, Craig R.¹ 

[1] Department of Radiology, University of Texas Southwestern Medical Center, Dallas, TX, USA;Department of Biochemistry, UT Southwestern Medical Center, Dallas, TX, USA

关键词: 13C isotopomer analysis;    Gluconeogenesis;    Pyruvate recycling;   

DOI  :  10.1016/S0014-5793(97)00764-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Simple equations that relate glucose and glutamate ¹³C-NMR multiplet areas to gluconeogenesis and pyruvate recycling during metabolism of [1,2,3-¹³C₃]propionate are presented. In isolated rat livers, gluconeogenic flux was 1.2 times TCA cycle flux and about 40% of the oxaloacetate pool underwent recycling to pyruvate prior to formation of glucose. The ¹³C spectra of glucose collected from rats after gastric versus intravenous administration of [1,2,3-¹³C₃]propionate indicated that pyruvate recycling was slightly higher in vivo (49%) while glucose production was unchanged. This indicates that a direct measure of gluconeogenesis and pyruvate recycling may be obtained from a single ¹³C-NMR spectrum of blood collected after oral administration of enriched propionate.

【 授权许可】

Unknown