ATR‐FTIR spectroscopic investigation of E. coli transconjugants β‐lactams‐resistance phenotype

FEBS Letters

Allouch, P⁴  Bloy, C³  Bouhedja, W²  Sockalingum, G.D²  Pina, P⁴  Labia, R¹  Manfait, M²  Millot, J.M² 

[1] CNRS, Unité UMR 175, 6 rue de l'Université, 29000 Quimper, France;Laboratoire de Spectroscopie Biomoléculaire, IFR 53, UFR de Pharmacie, Université de Reims Champagne-Ardenne, 51096 Reims, France;Laboratoires Cassenne, 95521 Cergy Pontoise, France;Service d'Hygiène Hospitalière, Hôpital Mignot, CH de Versailles, 78157 Le Chesnay, France

关键词: Escherichia coli K12;    TEM transconjugant;    SHV transconjugant;    ATR-FTIR spectroscopy;    Cluster analysis;   

DOI  :  10.1016/S0014-5793(97)00725-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Hyphenation of attenuated total reflection Fourier transform infrared spectroscopy and cluster analysis has been used to characterise a susceptible Escherichia coli K12 strain and the transconjugants TEM-1, TEM-2, TEM-3, SHV-2, SHV-3, SHV-4. A good discrimination of the susceptible strain from the transconjugants was obtained. Although a limited success was achieved in the differentiation of SHV and TEM phenotypes in general, results obtained with TEM-2 and SHV-3 were convincing. Spectral differences observed are ascribed to the global effects of the conjugation process, particularly their repercussions in the nucleic acids and carbohydrate absorbing regions, rather than to β-lactamase point-mutations.

【 授权许可】

Unknown