Continuous hepatocyte growth factor supply prevents lipopolysaccharide‐induced liver injury in rats

FEBS Letters

Imamura, Masayuki¹  Kasamatsu, Takayuki¹  Tanaka, Junji¹  Seto, Shin-ichi¹  Yamaoka, Shoji³  Funaki, Naomi¹  Kaido, Toshimi¹  Nakamura, Toshikazu² 

[1] Department of Surgery and Surgical Basic Science, Kyoto University School of Medicine, Shogoin Kawaracho Sakyo-ku, Kyoto 606-01, Japan;Division of Biochemistry, Biomedical Research Center, Osaka University Medical School, Suita, Osaka 565, Japan;Department of Viral Oncology, Institute for Virus Research, Kyoto University, Shogoin Kawaracho Sakyo-ku, Kyoto 606-01, Japan

关键词: Hepatocyte growth factor;    CCl4;    Endotoxin;    Liver injury;    Protection;    rHGF;    recombinant hepatocyte growth factor;    LPS;    lipopolysaccharide;    GOT;    glutamic oxalacetic transaminase;    GPT;    glutamic pyruvic transaminase;    BS;    blood sugar;    CCl4;    carbon tetrachloride;   

DOI  :  10.1016/S0014-5793(97)00744-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We present a rat model in which continuous supply of hepatocyte growth factor (HGF) prevents liver injury induced by carbon tetrachloride (CCl₄) and E. coli 011:B4 lipopolysaccharide (LPS). Rat fibroblasts genetically modified to secrete rat HGF were implanted in syngenic rat spleen 7 days before administration of the hepatotoxins. Rats with HGF-secreting fibroblasts in the spleen showed a dramatic resistance to CCl₄- and LPS-induced liver injury. In the LPS-induced liver injury model, blood chemical analysis revealed that the increase in serum glutamic oxalacetic transaminase level and the decrease in blood sugar level were remarkably suppressed in rats with HGF-secreting cells in the spleen. Most importantly, their survival rate was greatly improved compared to other control groups of rats. Thus our results indicate a new role of HGF in liver protection during endotoxemia and convey important clinical implications for developing new therapeutic modalities in the treatment of liver failure caused by endotoxemia.

【 授权许可】

Unknown