BIT, an immune antigen receptor‐like molecule in the brain

FEBS Letters

Omori, Akira¹  Sano, Shin-ichiro¹  Hasegawa, Junko¹  Ohnishi, Hiroshi¹  Kubota, Misae¹ 

[1] Mitsubishi Kasei Institute of Life Sciences, 11 Minamiooya, Machida, Tokyo 194, Japan

关键词: Immunoglobulin superfamily;    Immune antigen receptor-like molecule;    Tyrosine phosphorylation;    Neurite extension;    Rat brain;    TCR;    T-cell receptor;    MHC;    major histocompatibility complex;    TAM;    tyrosine-based activation motif;    SH2;    src homology 2;    IRS-1;    insulin receptor substrate-1;    PMSF;    phenylmethylsulfonyl fluoride;    HEPES;    N-2-hydroxyethylpiperazine-N′-ethanesulfonic acid;    CHAPS;    3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate;   

DOI  :  10.1016/S0014-5793(97)00724-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We previously found a brain-specific glycoprotein in the rat brain. It postnatally increases and is rich in the mature brain. We cloned cDNA of this protein. It is composed of a signal peptide, a V-type immunoglobulin domain, two C1-type immunoglobulin domains, a transmembrane segment and a cytoplasmic region containing two tyrosine-based activation motifs (TAM) that are variants of the antigen receptor signaling motifs. The overall structure is similar to those of immune antigen receptors. This molecule, BIT (brain immunoglobulin-like molecule with TAMs), is a major endogenous substrates of brain tyrosine kinases in vitro. Cerebral cortical neurons could extend their neurites on BIT-coated substrate and anti-BIT monoclonal antibody specifically inhibited the effect. These findings and our recent study concerning BIT signal transduction mechanism suggest that BIT, an immune antigen receptor-like molecule of the brain, functions as a membrane signaling molecule that may participate in cell–cell interaction.

【 授权许可】

Unknown