Differential expression of genes coding for ABC transporters after treatment of Arabidopsis thaliana with xenobiotics

FEBS Letters

Fromentau, Myriam¹  Schmid, Jürg²  Vogt, Esther²  Martinoia, Enrico²  Amrhein, Nikolaus²  Tommasini, Roberto² 

[1] Génétique Physiologique et Moléculaire, Université de Poitiers, 40 Avenue du Recteur Pineau, 86022 Poitiers, France;Institute of Plant Sciences, Swiss Federal Institute of Technology, Universitätstrasse 2, CH-8092 Zürich, Switzerland

关键词: ABC transporter;    Expression;    Xenobiotic;    Arabidopsis thaliana;    IRL 1803;    3-hydroxy-3-(2H-(1;    2;    4)triazole-3-yl)-cyclohexyl-phosphonic acid;    CDNB;    1-chloro-2;    4-dinitrobenzene;    PIPES;    1;    4-piperazinediethanesulfonic acid;    EST;    expressed sequence tags;    MRP;    multidrug-resistance-associated protein;    ABC;    ATP-binding cassette;   

DOI  :  10.1016/S0014-5793(97)00702-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

ATP-binding cassette (ABC) transporters are thought to be involved in many cellular detoxification mechanisms. Performing a BLAST search, we found four distinct expressed sequence tags (EST) of Arabidopsis thaliana highly similar to the human and fungal glutathione-conjugate ABC transporters. We studied the expression of the corresponding genes in response to various xenobiotics in an effort to gain information on their function. The abundance of transcripts corresponding to one of the genes (EST1) was not affected by the various compounds tested, whereas the abundance of transcripts corresponding to the other three genes (EST2, EST3, EST4) was increased by 1-chloro-2,4-dinitrobenzene, primisulfuron and IRL 1803. Treatment of Arabidopsis with either primisufuron or IRL 1803 resulted in a more than 40-fold increase in EST2-specific transcripts.

【 授权许可】

Unknown