Effect of anti‐apoptotic genes and peptide inhibitors on cytoplasmic acidification during apoptosis

FEBS Letters

Khar, Ashok¹  Pardhasaradhi, B.V.V¹  Srinivas, Usha K¹ 

[1] Centre for Cellular and Molecular Biology, Uppal Road, Hyderabad 500 007, India

关键词: Tumor cell apoptosis;    pH reduction;    Anti-apoptotic gene;    Peptide inhibitor;   

DOI  :  10.1016/S0014-5793(97)00665-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Cytoplasmic acidification has been shown to occur during the apoptotic process of cell death although its relation with other events in the process are not yet clear. AK-5 tumor cells have been shown to undergo apoptosis upon treatment with stimuli like dexamethasone (1 μM) or with serum from animals that reject AK-5 tumor. The current study was designed to measure the extent of cytoplasmic acidification during apoptosis in AK-5 cells and to study the effect of antiapopoptic genes and peptide inhibitors on cytoplasmic acidification. Our results show that AK-5 cells when triggered into apoptosis show intracellular acidification by about 0.2 pH units and this is prevented when cells are treated with peptide inhibitors. In addition cytoplasmic acidification does not occur when AK-5 cells are transfected with anti-apoptotic genes Nedd-2 A.S, Crm A or bcl-2 which inhibit apoptosis.

【 授权许可】

Unknown