Advanced glycation endproducts stimulate the MAP‐kinase pathway in tubulus cell line LLC‐PK1

FEBS Letters

Heidland, August¹  Simm, Andreas³  Seif, Farshid³  Richter, Hedwig²  Schinzel, Reinhard³  Münch, Gerald³  Vamvakas, Spiridou²  Schenk, Oliver¹ 

[1] Kuratorium für Dialyse und Nierentransplantation, Hans Brandmann Weg 1, 97078 Würzburg, FRG;Institut für Toxikologie, Versbacher Str., 97078 Würzburg, FRG;Physiologische Chemie, Theodor-Boveri-Institut der Universität Würzburg, Am Hubland, 97074 Würzburg, FRG

关键词: Advanced glycation endproducts;    MAP-Kinase;    Tubulus;   

DOI  :  10.1016/S0014-5793(97)00644-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Advanced glycation endproducts (AGEs) are suggested to play an important role in diabetic nephropathy. They induce specific cellular responses such as the release of cytokines in different cell lines. The effect of AGEs on signal transduction pathways was investigated in the renal tubulus cell line LLC-PK₁. Using a serine-phosphate-specific antibody AGE-induced cellular responses associated with phosphorylation/dephosphorylation events were demonstrated. In particular, the p42^MAP kinase and its downstream target, the AP-1 complex, are shown to be activated by AGE-BSA but not by BSA. In contrast, only partial phosphorylation is observed for the p70^S6-kinase. Thus, AGEs appear to induce specific signal transduction pathways.

【 授权许可】

Unknown