Decrease of rotenone inhibition is a sensitive parameter of complex I damage in brain non‐synaptic mitochondria of aged rats

FEBS Letters

Biagini, Graziella¹  Lenaz, Giorgio⁴  Pallotti, Francesco²  Viticchi, Claudio³  Luisa Genova, Maria⁴  Marchetti, Mario⁴  Federico Villa, Roberto⁵  Tietz, Catleen²  Bovina, Carla⁴  Gorini, Antonella⁵  Pugnaloni, Armanda¹ 

[1]Institute of Human Morphology, University of Ancona, via Tronto 10/a, 60020 Torrette di Ancona, Italy

[2]Institute of Histology, University of Bologna, via Belmeloro 8, 40126 Bologna, Italy

[3]INRCA, via S. Margherita 5, 60124 Ancona, Italy

[4]Department of Biochemistry, University of Bologna, via Irnerio 48, 40126 Bologna, Italy

[5]Institute of Pharmacology, University of Pavia, p.zza Botta 11, 27100 Pavia, Italy

关键词: Aging;    Brain mitochondria;    Complex I;    Rotenone;    Mitochondrial DNA;   

DOI  :  10.1016/S0014-5793(97)00638-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We investigated NADH oxidation in non-synaptic and synaptic mitochondria from brain cortex of 4- and 24-month-old rats. The NADH oxidase activity was significantly lower in non-synaptic mitochondria from aged rats; we also found a significant decrease of sensitivity of NADH oxidation to the specific Complex I inhibitor, rotenone. Since the rotenone-binding site encompasses Complex I subunits encoded by mtDNA, these results are in accordance with the mitochondrial theory of aging, whereby somatic mtDNA mutations are at the basis of cellular senescence. Accordingly, a 5 kb deletion was detected only in the cortex of the aged animals.

【 授权许可】

Unknown