Dramatic enhancement of the specific expression of the heart‐type fatty acid binding protein in rat brown adipose tissue by cold exposure

FEBS Letters

Daikoku, Takiko¹  Shinohara, Yasuo¹  Terada, Hiroshi¹  Shima, Atsushi²  Yamazaki, Naoshi¹ 

[1] Faculty of Pharmaceutical Sciences, University of Tokushima, Tokushima 770, Japan;Nutrition Research Institute, Otsuka Pharmaceutical Factory, Inc., Naruto 772, Japan

关键词: Brown adipose tissue;    Energy metabolism;    Fatty acid binding protein (FABP);    Northern blot analysis;    WAT;    white adipose tissue;    BAT;    brown adipose tissue;    UCP;    uncoupling protein;    CPT;    carnitine palmitoyltransferase;    CPTI-L;    liver-type carnitine palmitoyltransferase I;    CPTI-M;    muscle-type carnitine palmitoyltransferase I;    FABP;    fatty acid binding protein;    DMEM;    Dulbecco's modified Eagle's medium;    NE;    norepinephrine;    RT-PCR;    reverse transcription followed by the polymerase chain reaction;    G3PDH;    glycerol 3-phosphate dehydrogenase;   

DOI  :  10.1016/S0014-5793(97)00619-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

To understand the difference in energy metabolisms in brown (BAT) and white (WAT) adipose tissues, we examined the steady-state transcript levels of the heart-type and adipose-type fatty acid binding proteins (H-FABP and A-FABP, respectively) by Northern blot analysis. The transcript of H-FABP in rat BAT was increased about 100-fold by cold exposure, whereas that in WAT was negligible, and was increased only slightly by cold exposure. The transcript of A-FABP was observed in both BAT and WAT, the level being slightly greater in WAT. However, its transcript level was not affected by cold exposure in either adipose tissue. In addition, on treatment with norepinephrine (NE), transcript level of H-FABP was elevated markedly but that of A-FABP was not changed in rat brown adipocytes. Therefore, the stimulatory effect of cold exposure on the transcript of H-FABP in BAT was concluded to be mediated by NE, like that of the uncoupling protein (UCP). Thus, the expressions of H-FABP and UCP may be controlled by the same mechanism.

【 授权许可】

Unknown