Cytosolic NAD+ content strictly depends on ATP concentration in isolated liver cells

FEBS Letters

Guérin, Bernard¹  Rigoulet, Michel¹  Devin, Anne¹ 

[1] Institut de Biochimie et Génétique Cellulaires du CNRS, Université de Bordeaux 2, 1 rue Camille Saint-Saëns, 33 077 Bordeaux cedex, France

关键词: Isolated hepatocyte;    Adenine nucleotide;    Pyrimidine nucleotide;    Metabolism;   

DOI  :  10.1016/S0014-5793(97)00612-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

By focusing on the question of the thermodynamic relationships involved in the regulation of biological energy conversion, bioenergetic studies usually consider the free pyridine and adenine nucleotide rather than their total pools, in either cytosol or mitochondria. In this study, we report a new observation that, at steady state, nicotinamide nucleotide content is increased by a rise in the ATP content of the whole cell under physiological conditions. It is a straight line relationship when only NAD⁺ and ATP are considered. When regarding the compartmentation of this phenomenon, it appears that the linear relationship between [NAD⁺] and [ATP] occurs only in the cytosol. Such a dependence could be a supplementary mechanism of regulation between various metabolic pathways in the liver cell.

【 授权许可】

Unknown