Identification of a biological inactive complex form of pokeweed antiviral protein

FEBS Letters

Jouvenot, Michèle¹  Poyet, Jean-Luc¹  Adami, Pascale¹  Desvoyes, Bénédicte¹  Schlick, Jean-Luc¹  Dulieu, Philippe¹ 

[1] Laboratoire de Biochimie et Biologie Moléculaire, UFR Sciences et Techniques, 16 route de Gray, 25030 Besançon Cedex, France

关键词: Phytolacca americana;    Pokeweed antiviral protein;    Ribosome inactivating protein;    Toxin inhibitor;    mAb;    monoclonal antibody;    E40;    pokeweed leaf extract after ammonium sulfate fractionation;    PE extract;    pokeweed leaf extract after preparative native gel electrophoresis;    ND-PAGE;    non-denaturing polyacrylamide gel electrophoresis;   

DOI  :  10.1016/S0014-5793(97)00648-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Pokeweed antiviral protein (PAP) inactivates both eukaryotic and prokaryotic ribosomes via a specific depurination of rRNA. The sensitivity of pokeweed ribosomes to PAP implies the existence of a mechanism to protect the plant. Using monoclonal antibodies specific to PAP, a protein complex (PAPi) which contained PAP was identified in leaf extract. In this complex, the enzymatic activity of the toxin was strongly inhibited. This protein complex had a pI lower than that of PAP and was separated from free PAP by a preparative native gel electrophoresis. PAPi had an apparent molecular mass of 57 kDa and was dissociated by heating for 5 min at 80°C or by treatment by alkaline or acidic pH or by 7 M urea. The other components involved in the complex remain unknown.

【 授权许可】

Unknown