Purification and structure of mutacin B‐Ny266: a new lantibiotic produced by Streptococcus mutans

FEBS Letters

Mota-Meira, Marilaine¹  Lavoie, Marc C.²  LaPointe, Gisèle¹  Lacroix, Christophe¹ 

[1]Centre de Recherche en Sciences et Technologie du Lait (STELA), Faculté des Sciences de l'Agriculture et de l'Alimentation, Université Laval, Québec, Canada

[2]Département de Biochimie, Faculté des Sciences et de Génie, Université Laval, Québec, Québec G1K 7P4), Canada

关键词: Mutacin;    Bacteriocin;    Lantibiotic;    Streptococcus mutans;    Amino acid sequence;    Abu;    2-aminobutyric acid;    ATCC;    American Type Culture Collection;    AU;    arbitrary units;    BM-Lan;    β-methyllanthionine;    BMSEC;    β-methyl-S-ethylcysteine;    dhA or U;    2;    3-didehydroalanine;    dhB or O;    (2)-2;    3-didehydrobutyrine;    DSER;    PTH-dithiothreitol adduct of dhA;    HPLC;    high-pressure liquid chromatography;    Lan;    lanthionine;    OPA;    o-phthaldialdehyde;    PTH;    phenylthiohydantoin;    RP-HPLC;    reversed-phase high-pressure liquid chromatography;    SEC;    S-ethylcysteine;    TFA;    trifluoroacetic acid;    TSAYE;    trypticase soy agar yeast extract;   

DOI  :  10.1016/S0014-5793(97)00425-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Mutacins are bactericidal substances of proteinaceous nature produced by Streptococcus mutans. Lantibiotics are antibacterial substances containing post-translationally modified amino acids such as lanthionine. Mutacin B-Ny266 was purified from the cell pellet of S. mutans strain Ny266 by ethanol extraction at pH 2.0 followed by reversed-phase chromatography (Sep-Pak® cartridge) and by HPLC on a C₁₈ column. The mean purification factor was 3240±81 and the mean yield was 1.0±0.1%. Molecular mass of mutacin B-Ny266 as determined by mass spectroscopy is 2270.29±0.21 Da. The amino acid sequence of the purified active fraction was obtained by Edman degradation after treatment with alkaline ethanethiol. Twenty-one amino acids were detected in this analysis. Mutacin B-Ny266 belongs to the type A lantibiotics. The proposed sequence is: F–K––W–U–F–––P–G––A–K–O–G––F–N––Y–. The molecule differs from that of epidermin/staphylococcin 1580 and gallidermin at positions 1, 2, 4, 5 and 6.

【 授权许可】

Unknown