Medium‐reductant directed expression of methyl coenzyme M reductase isoenzymes in Methanobacterium thermoautotrophicum (strain ΔH)

FEBS Letters

van der Drift, Chris¹  de Wijs, Jeroen L.J¹  Keltjens, Jan T¹  Pennings, Jeroen L.A¹ 

[1] Department of Microbiology, University of Nijmegen, P.O. Box 9010, NL-6500 GL Nijmegen, The Netherlands

关键词: Methanogenesis;    Chemostat;    Transcriptional regulation;    Methyl coenzyme M reductase;    Methylene tetrahydomethanopterin dehydrogenase;    (Methanobacterium thermoautotrophicum);    DIG;    digoxigenin;    MCR;    methyl coenzyme M reductase;    MDH;    methylenetetrahydromethanopterin dehydrogenase;   

DOI  :  10.1016/S0014-5793(97)00595-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Methanobacterium thermoautotrophicum was grown in a chemostat under various controlled conditions in the presence of either sodium sulfide or sodium thiosulfate. After establishment of the steady state, cells were taken and examined for expression of the mRNA transcripts coding for the different forms of methyl coenzyme M reductase (MCR) and methylene tetrahydomethanopterin dehydrogenase (MDH). MCR isoenzyme II expression varied most markedly. Expression was found not only to depend on known parameters temperature, pH and gassing rate, but also on the medium composition, especially the reductant present.

【 授权许可】

Unknown