Iron triggers a rapid induction of ascorbate peroxidase gene expression in Brassica napus

FEBS Letters

Fourcroy, Pierre²  Inzé, Dirk³  Vansuyt, Gérard²  Briat, Jean-Francois²  Lopez, Félicie¹ 

[1]Instituto de Biologia Molecular y Cellular de Plantas, Universidad Polytecnica de Valencia, CSIC, Camino de Vera, E-46022 Valencia, Spain

[2]Biochimie et Physiologie Moléculaire des Plantes, CNRS URA 2133, ENSAM-INRA, 2 place Viala, F-34060 Montpellier Cedex 1, France

[3]VIB, Universiteit Gent, Laboratorium voor Genetica, B-9000 Gent, Belgium

关键词: Iron;    Ascorbate peroxidase;    Oxidative stress;    Brassica napus;    ABA;    abscisic acid;    APX;    ascorbate peroxidase;    DTT;    dithiothreitol;    NAC;    N-acetyl cysteine;    SOD;    superoxide dismutase;   

DOI  :  10.1016/S0014-5793(97)00587-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

In plants, only ferritin gene expression has been reported to be iron-dependent. Here it is demonstrated that an iron overload of Brassica napus seedlings causes a large and rapid accumulation of ascorbate peroxidase transcripts, a plant-specific hydrogen peroxide-scavenging enzyme. This result documents a novel link between iron metabolism and oxidative stress. The ascorbate peroxidase mRNA abundance was not modified by reducing agents like N-acetyl cysteine, glutathione and ascorbate or by pro-oxidants such as hydrogen peroxide or diamide. Furthermore, the iron-induced ascorbate peroxidase mRNA accumulation was not antagonized by N-acetyl cysteine. Abscisic acid had no effect on the ascorbate peroxidase gene expression. Taken together these results suggest that iron-mediated expression of ascorbate peroxidase gene occurs through a signal transduction pathway apparently different from those already described for plant genes responsive to oxidative stress.

【 授权许可】

Unknown