Exogenous, but not endogenous, nitric oxide increases proliferation rates in senescent human fibroblasts

FEBS Letters

Gansauge, Susanne¹  Rau, Bettina¹  Poch, Bertram¹  Beger, Hans G¹  Schoenberg, Michael H¹  Gansauge, Frank¹  Nussler, Andreas K¹ 

[1] Division of Molecular Oncology, Department of General Surgery, University of Ulm, Steinhövelstr. 9, 89075 Ulm, Germany

关键词: Fibroblast;    Human;    Nitric oxide;    Proliferation;   

DOI  :  10.1016/S0014-5793(97)00544-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We investigated the effects of endogenously produced and exogenously applied nitric oxide (NO) on cell proliferation rates and cell cycle regulation in senescent human fibroblasts (WI38). Induction of inducible nitric oxide synthase by tumor necrosis factor-α, interferon-γ and interleukin-1β inhibited cell proliferation and led to a G1 arrest. These effects were partially reversible by N ^G-monomethyl-arginine (NMA). Addition of the NO donors sodium nitroprusside (SNP) or S-nitroso-N-acetylpenicillamine (SNAP) increased cell proliferation rates as well as the S/G2 fraction. This points to a functional role of NO in cell cycle regulation and cell proliferation in human fibroblasts which depends on the mode of NO generation as well as the culture conditions used.

【 授权许可】

Unknown