Palytoxin effects through interaction with the Na,K‐ATPase in Xenopus oocyte

FEBS Letters

Wang, Xinyu¹  Horisberger, Jean-Daniel¹ 

[1] Institute of Pharmacology and Toxicology, University de Lausanne, Rue du Bugnon 27, CH-1005 Lausanne, Switzerland

关键词: Na;    K-ATPase;    Palytoxin;    Ouabain;    Single-channel current;   

DOI  :  10.1016/S0014-5793(97)00559-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Palytoxin (PTX) is known to bind to Na,K-ATPase, to inhibit its activity, and to induce cation conductance, but the mechanism of these effects is still poorly understood. In Xenopus oocytes, PTX induced a large cation conductance, an effect that could be prevented or reversed by ouabain for oocytes expressing Xenopus Na,K-pumps but not with those expressing Bufo Na,K-pumps. In both cases patch-clamp experiments demonstrated a 7–8 pS channel in the presence of PTX. A large PTX-induced conductance could be observed with minimal Na,K-pump inhibition. From the single PTX-induced channel and macroscopic whole oocyte conductance, and the number of Na,K-pumps, we can conclude that PTX-induced conductance occurs through a direct interaction of PTX with a small number of Na,K-pumps.

【 授权许可】

Unknown