Inhibition of Galβ1,4GlcNAc α2,6 sialyltransferase expression by antisense‐oligodeoxynucleotides

FEBS Letters

Schlag, Peter M¹  Hohaus, Kathrin¹  Kemmner, Wolfgang¹ 

[1] Department of Surgical Oncology, Robert-Rössle-Klinik at the Max-Delbrück-Centrum for Molecular Medicine, Robert-Rössle-Str. 10, D-13122 Berlin, Germany

关键词: Galβ1;    4GlcNAc α2;    6 sialyltransferase;    Antisense-oligodeoxynucleotides;    Cell surface glycoconjugates;    Colorectal carcinomas;    Metastasis;   

DOI  :  10.1016/S0014-5793(97)00543-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Treatment of human colorectal carcinoma cells HT29 with specific antisense oligodeoxynucleotides led to a decreased Galβ1,4GlcNAc α2,6 sialyltransferase activity on the level of protein expression as well as on the mRNA level. Antisense treatment did not effect cell viability or cell growth. Oligodeoxynucleotides which were complementary to the region upstream of the initiation codon were particularly effective in inhibition of enzyme expression. No such inhibition was found by treatment of cells with oligodeoxynucleotides complementary to the region downstream of the initiation codon or by treatment of cells with scrambled controls or sense oligodeoxynucleotides.

【 授权许可】

Unknown