The nodulation gene nolK of Azorhizobium caulinodans is involved in the formation of GDP‐fucose from GDP‐mannose

FEBS Letters

Holsters, Marcelle¹  Mergaert, Peter¹  Van Montagu, Marc¹ 

[1] Laboratorium voor Genetica, Department of Genetics, Flanders Interuniversity Institute for Biotechnology (VIB), Universiteit Gent, K.L. Ledeganckstraat 35, B-9000 Gent, Belgium

关键词: Fucosylation;    Nod factor;    nod gene;    Rhizobium;    RP;    reverse phase;    TLC;    thin-layer chromatography;   

DOI  :  10.1016/S0014-5793(97)00461-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The nolK gene of Azorhizobium caulinodans is essential for the incorporation of a fucosyl group in Nod factors. A NAD(P)-binding site is present in the NolK amino acid sequence and the gene is homologous to Escherichia coli genes, presumably involved in GDP-fucose synthesis. Protein extracts of A. caulinodans, overexpressing nolK, have an enzyme activity that synthesizes GDP-fucose from GDP-mannose. nolK most probably encodes a 4-reductase performing the last step in GDP-fucose synthesis. Wild-type A. caulinodans produces a population of fucosylated and non-fucosylated molecules but the nolK-overexpressing strain produces only fucosylated Nod factors. Thus, the production of activated fucosyl donors is a rate-limiting step in Nod factor fucosylation.

【 授权许可】

Unknown