Mycothiol, 1‐O‐(2′‐[N‐acetyl‐L‐cysteinyl]amido‐2′‐deoxy‐α‐D‐glucopyranosyl)‐D‐myo‐inositol, is the factor of NAD/factor‐dependent formaldehyde dehydrogenase

FEBS Letters

Misset-Smits, Marijke³  van Ophem, Peter W²  Sakuda, Shohei¹  Duine, Johannis A³ 

[1] Department of Applied Biological Chemistry, The University of Tokyo, Tokyo, Japan;Department of Biology, Northeastern University, Boston, USA;Department of Microbiology and Enzymology, Delft University of Technology, 2628 BC Delft, The Netherlands

关键词: Mycothiol;    Formaldehyde dehydrogenase;    Glutathione;    Actinomycetes;    Alcohol dehydrogenase class III;    MySH;    mycothiol (1-O-(2′-[N-acetyl-L-cysteinyl]amido-2′-deoxy-α-D-glucopyranosyl)-D-myo-inositol);    MySSyM;    the disulfide form of MySH;    GD-FAlDH;    NAD/glutathione-dependent formaldehyde dehydrogenase;    MD-(formerly FD-)-dependent FAlDH;    NAD/mycothiol-dependent formaldehyde dehydrogenase;    DTT;    dithiothreitol;   

DOI  :  10.1016/S0014-5793(97)00510-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Two different NAD/coenzyme-dependent formaldehyde dehydrogenases exist, the well-known NAD/GSH-dependent (EC 1.2.1.1) and the more recently discovered NAD/Factor-dependent enzyme. The GSH-dependent one has been found in eukaryotes and Gram-negative bacteria, the Factor-dependent one in two different Gram-positive bacteria. Previous work also showed that Factor and GSH are not interchangeable in the enzymatic reactions. Here it is revealed that the Factor is identical to mycothiol (MySH), 1-O-(2′-[N-acetyl-L-cysteinyl]amido-2′-deoxy-α-D-glucopyranosyl)-D-myo-inositol, a thiol compound which has recently been detected in Actinomycetes. Thus, MySH is GSH's companion as it is the coenzyme for the enzyme which henceforth can be indicated as NAD/MySH-dependent formaldehyde dehydrogenase.

【 授权许可】

Unknown