FEBS Letters | |
Mycothiol, 1‐O‐(2′‐[N‐acetyl‐L‐cysteinyl]amido‐2′‐deoxy‐α‐D‐glucopyranosyl)‐D‐myo‐inositol, is the factor of NAD/factor‐dependent formaldehyde dehydrogenase | |
Misset-Smits, Marijke3  van Ophem, Peter W2  Sakuda, Shohei1  Duine, Johannis A3  | |
[1] Department of Applied Biological Chemistry, The University of Tokyo, Tokyo, Japan;Department of Biology, Northeastern University, Boston, USA;Department of Microbiology and Enzymology, Delft University of Technology, 2628 BC Delft, The Netherlands | |
关键词: Mycothiol; Formaldehyde dehydrogenase; Glutathione; Actinomycetes; Alcohol dehydrogenase class III; MySH; mycothiol (1-O-(2′-[N-acetyl-L-cysteinyl]amido-2′-deoxy-α-D-glucopyranosyl)-D-myo-inositol); MySSyM; the disulfide form of MySH; GD-FAlDH; NAD/glutathione-dependent formaldehyde dehydrogenase; MD-(formerly FD-)-dependent FAlDH; NAD/mycothiol-dependent formaldehyde dehydrogenase; DTT; dithiothreitol; | |
DOI : 10.1016/S0014-5793(97)00510-3 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
Two different NAD/coenzyme-dependent formaldehyde dehydrogenases exist, the well-known NAD/GSH-dependent (EC 1.2.1.1) and the more recently discovered NAD/Factor-dependent enzyme. The GSH-dependent one has been found in eukaryotes and Gram-negative bacteria, the Factor-dependent one in two different Gram-positive bacteria. Previous work also showed that Factor and GSH are not interchangeable in the enzymatic reactions. Here it is revealed that the Factor is identical to mycothiol (MySH), 1-O-(2′-[N-acetyl-L-cysteinyl]amido-2′-deoxy-α-D-glucopyranosyl)-D-myo-inositol, a thiol compound which has recently been detected in Actinomycetes. Thus, MySH is GSH's companion as it is the coenzyme for the enzyme which henceforth can be indicated as NAD/MySH-dependent formaldehyde dehydrogenase.
【 授权许可】
Unknown
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