FEBS Letters | |
Structure of in‐serum transfecting DNA–cationic lipid complexes | |
Aguerre-Chariol, O4  Boukhnikachvili, T3  Vacus, J1  Airiau, M4  Lesieur, S2  Ollivon, M2  | |
[1] Rhône-Poulenc Rorer, Centre de Recherche de Vitry-Alfortville 13, Quai Jules Guesde, 94400 Vitry sur Seine, France;URA-CNRS 1218, Faculté de Pharmacie Paris sud 5, rue J.B. Clément, 92296 Châtenay Malabry cedex, France;Rhône-Poulenc Rorer Gencell, Centre de Recherche de Vitry-Alfortville 13, Quai Jules Guesde, 94400 Vitry sur Seine, France;Rhône-Poulenc 52, rue de la Haie Coq, 93308 Aubervilliers, France | |
关键词: Transfection efficiency; Cationic lipid; DNA condensation; Cryo-transmission electron microscopy; X-ray diffraction; Lamellar structure; | |
DOI : 10.1016/S0014-5793(97)00505-X | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
Noticeable modifications of in-serum transfection efficiency of dioctadecylamidoglycyl-spermine (DOGS)–DNA complexes are observed, depending on DNA condensation conditions. The structures of the complexes are studied, keeping in mind the variability of lipid polymorphism, by cryo-transmission electron microscopy and X-ray diffraction. By increasing both pH and ionic strength, well-organised lamellar structures with a period of 65 Å replace supramicellar aggregates. A relationship between the structures and their in-vitro transfection activity is established. Efficiency in the presence of serum is maintained when a lamellar arrangement is involved.
【 授权许可】
Unknown
【 预 览 】
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