Structure of in‐serum transfecting DNA–cationic lipid complexes

FEBS Letters

Aguerre-Chariol, O⁴  Boukhnikachvili, T³  Vacus, J¹  Airiau, M⁴  Lesieur, S²  Ollivon, M² 

[1] Rhône-Poulenc Rorer, Centre de Recherche de Vitry-Alfortville 13, Quai Jules Guesde, 94400 Vitry sur Seine, France;URA-CNRS 1218, Faculté de Pharmacie Paris sud 5, rue J.B. Clément, 92296 Châtenay Malabry cedex, France;Rhône-Poulenc Rorer Gencell, Centre de Recherche de Vitry-Alfortville 13, Quai Jules Guesde, 94400 Vitry sur Seine, France;Rhône-Poulenc 52, rue de la Haie Coq, 93308 Aubervilliers, France

关键词: Transfection efficiency;    Cationic lipid;    DNA condensation;    Cryo-transmission electron microscopy;    X-ray diffraction;    Lamellar structure;   

DOI  :  10.1016/S0014-5793(97)00505-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Noticeable modifications of in-serum transfection efficiency of dioctadecylamidoglycyl-spermine (DOGS)–DNA complexes are observed, depending on DNA condensation conditions. The structures of the complexes are studied, keeping in mind the variability of lipid polymorphism, by cryo-transmission electron microscopy and X-ray diffraction. By increasing both pH and ionic strength, well-organised lamellar structures with a period of 65 Å replace supramicellar aggregates. A relationship between the structures and their in-vitro transfection activity is established. Efficiency in the presence of serum is maintained when a lamellar arrangement is involved.

【 授权许可】

Unknown