The transcription of NAM7/UPF1 is enhanced in the absence of Cyp1p/Hap1p concomitant with the appearance of an ISF1‐NAM7 cotranscript in Saccharomyces cerevisiae

FEBS Letters

de Pinto, Brigida¹  Altamura, Nicola¹  Verdiére, Jacqueline²  Castaldo, Rosa¹ 

[1] Centro di Studio sui Mitocondri e Metabolismo Energetico, CNR, presso Università di Bari, via Amendola 165/A, 70126 Bari, Italy;Centre de Génétique Moléculaire du CNRS, Laboratoire propre associé à l'Université Pierre et Marie Curie, F-91198 Gif-sur-Yvette, Cedex, France

关键词: Mitochondrial function;    Nuclear gene;    CYP1(HAP1);    Cotranscription;    Transcriptional regulation;   

DOI  :  10.1016/S0014-5793(97)00497-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The two adjacent nuclear genes ISF1 and NAM7 cooperatively participate in mitochondrial functions. It is well known that Cyp1p(Hap1p) activates a number of genes involved in these same functions. We show in this paper that Cyp1p influences the transcriptional regulation of NAM7. In addition, a significant amount of ISF1–NAM7 cotranscript is observed in a cyp1 mutant context. An extensive analysis of the intergenic region which separates the two genes revealed 5′ starts of the NAM7 transcripts, additional to those previously mapped. These new 5′ starts overlap the 3′ ends of ISF1. We propose that NAM7 is under the control of a negative Cyp1p-dependent regulator and that its absence favours a transcriptional read-through which results in the ISF1–NAM7 cotranscript we have identified.

【 授权许可】

Unknown