Analysis of phosphofructokinase subunits and isozymes in ascites tumor cells and its original tissue, murine mammary gland

FEBS Letters

J. Aragón, Juan¹  Sánchez-Martı́nez, Cristina¹ 

[1] Departamento de Bioquı́mica de la UAM and Instituto de Investigaciones Biomédicas del CSIC, Facultad de Medicina, Universidad Autónoma de Madrid, Arzobispo Morcillo 4, 28019 Madrid, Spain

关键词: Phosphofructokinase;    Fructose-1;    6-bisphosphate;    Glycolysis;    Allosteric regulation;    Ascites tumor cell;    Mammary gland;   

DOI  :  10.1016/S0014-5793(97)00496-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Phosphofructokinase (PFK) subunits and isozymes have been examined in ascites tumor cells and murine mammary gland, the tissue from where this tumor originated. Ascites tumor was found to contain predominantly the C-type subunit, whereas the L-type subunit was more abundant in mammary gland. An altered M-type subunit of lower electrophoretic mobility was found in both cell types and no M₄ homotetramers were detected in either of them. Characteristic regulatory properties of ascites tumor PFK, i.e. insensitivity to fructose-1,6-P₂ activation and inhibition by P-enolpyruvate, were also observed in the mammary gland isozyme. The nature of these properties and the contribution of the distinct subunit types to fructose-1,6-P₂ activation are discussed.

【 授权许可】

Unknown