Calcium‐ and pH‐linked oligomerization of sorcin causing translocation from cytosol to membranes

FEBS Letters

Vecchini, Paola¹  Zamparelli, Carlotta¹  Chiancone, Emilia¹  Verzili, Daniela¹  Ilari, Andrea¹ 

[1] CNR Center of Molecular Biology, Department of Biochemical Sciences ‘A. Rossi Fanelli’, Università La Sapienza, 00185 Rome, Italy

关键词: Sorcin;    Calcium- and pH-dependent conformational change;    Oligomerization;    Quin 2;    2-[[2-[bis-(carboxy-methyl)amino]-5-methyl-phenoxy]methyl]-6-methoxy-8[bis(carboxymethyl)amino]quinoline;    EGTA;    ethylene glycol bis(β-aminoethyl ether);   

DOI  :  10.1016/S0014-5793(97)00464-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Sorcin, a cytosolic calcium-binding protein containing a pair of EF-hand motifs, undergoes a Ca²⁺-dependent translocation to the cell membrane. The underlying conformational change is similar at pH 6.0 and 7.5 and consists in an increase in overall hydrophobicity that involves the aromatic residues and in particular the two tryptophan residues which become less exposed to solvent. The concomitant association from dimers to tetramers indicates that the tryptophan residues, which are located between the EF-hand sites, become buried at the dimer–dimer interface. Ca²⁺-bound sorcin displays a striking difference in solubility as a function of pH that has been ascribed to the formation of calcium-stabilized aggregates.

【 授权许可】

Unknown