Microinjected cDNA encoding JAK2 protein‐tyrosine kinase induces DNA synthesis in NIH 3T3 cells

FEBS Letters

Duhé, Roy J.²  Smith, Mark R.²  Farrar, William L.¹  Liu, Ya-lun² 

[1] Cytokine Molecular Mechanisms Section, Laboratory of Molecular Immunoregulation, National Cancer Institute - Frederick Cancer Research and Development Center, P.O. Box B, Frederick, MD 21702-1201, USA;Intramural Research Support Program, Science Applications International Corporation Frederick, National Cancer Institute - Frederick Cancer Research and Development Center, P.O. Box B, Frederick, MD 21702-1201, USA

关键词: Proto-oncogene;    Microinjection;    Protein-tyrosine kinase;    Janus kinase;    Tyrphostin AG-490;   

DOI  :  10.1016/S0014-5793(97)00456-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Microinjection of expression plasmids encoding either JAK2 or hyperactive (NΔ661)rJAK2 into serum-starved NIH 3T3 cells resulted in 20–30-fold induction of DNA synthesis. Control microinjections of buffer or parental pcDNA3 vector resulted in only 3–5-fold induction of DNA synthesis. Induction of DNA synthesis was blocked when plasmid encoding JAK2 was microinjected in the presence of the JAK2-selective inhibitor AG-490, whereas AG-490 did not block DNA synthesis induced by microinjected plasmid encoding (NΔ661)rJAK2. The ability of JAK2 to initiate the G_o/S cell cycle transition is comparable to that of other proto-oncogenes, and supports a mechanistic role for overexpressed Janus kinases in carcinogenesis.

【 授权许可】

Unknown