FEBS Letters | |
Microinjected cDNA encoding JAK2 protein‐tyrosine kinase induces DNA synthesis in NIH 3T3 cells | |
Duhé, Roy J.2  Smith, Mark R.2  Farrar, William L.1  Liu, Ya-lun2  | |
[1] Cytokine Molecular Mechanisms Section, Laboratory of Molecular Immunoregulation, National Cancer Institute - Frederick Cancer Research and Development Center, P.O. Box B, Frederick, MD 21702-1201, USA;Intramural Research Support Program, Science Applications International Corporation Frederick, National Cancer Institute - Frederick Cancer Research and Development Center, P.O. Box B, Frederick, MD 21702-1201, USA | |
关键词: Proto-oncogene; Microinjection; Protein-tyrosine kinase; Janus kinase; Tyrphostin AG-490; | |
DOI : 10.1016/S0014-5793(97)00456-0 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
Microinjection of expression plasmids encoding either JAK2 or hyperactive (NΔ661)rJAK2 into serum-starved NIH 3T3 cells resulted in 20–30-fold induction of DNA synthesis. Control microinjections of buffer or parental pcDNA3 vector resulted in only 3–5-fold induction of DNA synthesis. Induction of DNA synthesis was blocked when plasmid encoding JAK2 was microinjected in the presence of the JAK2-selective inhibitor AG-490, whereas AG-490 did not block DNA synthesis induced by microinjected plasmid encoding (NΔ661)rJAK2. The ability of JAK2 to initiate the Go/S cell cycle transition is comparable to that of other proto-oncogenes, and supports a mechanistic role for overexpressed Janus kinases in carcinogenesis.
【 授权许可】
Unknown
【 预 览 】
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