Aluminium fluoride associates with the small guanine nucleotide binding proteins

FEBS Letters

Mittal, Rohit²  Hall, Alan¹  Ahmadian, Mohammad Reza²  Wittinghofer, Alfred² 

[1]MRC Laboratory for Molecular Cell Biology, University College London, London WC1E 6BT, UK

[2]Max-Planck-Institut für molekulare Physiologie, Rheinlanddamm 201, 44139 Dortmund, Germany

关键词: Aluminium fluoride;    Ras;    Cdc42;    Rap;    Ran;   

DOI  :  10.1016/S0014-5793(97)00422-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

AlF₄ ⁻ has long been known to associate with and activate the GDP-bound alpha subunits of heterotrimeric G-proteins. Recently the small guanine nucleotide binding protein Ras has also been shown to associate with AlF₄ ⁻ in the presence of stoichiometric amounts of itsTPasectivatingrotein (GAP). Here we present the isolation of a stable Ras·GDP·AlF₄ ⁻·GAP ternary complex by gel filtration. In addition, we generalise the association of AlF₄ ⁻ with the small GTP-binding proteins by demonstrating ternary complex formation for the Cdc42, Rap and Ran proteins in the presence of their respective GAP proteins.

【 授权许可】

Unknown